MAB3D ATLAS

Our aim is to create a three-dimensional whole-brain reference atlas of protein expression in the adult mouse brain. This atlas will reveal underlying brain architecture and provide a platform for scientists to examine cellular and organ level changes in biomedical research and diagnosis... Toward this end, we are actively building a reference list of monoclonal antibodies compatible with immunohistochemistry and the iDISCO family of whole-mount methods. We use these antibodies to spatially profile the expression of diverse proteins at cellular resolution in the mouse brain in both 2D and 3D. To facilitate translational insights across species, we further characterize many of these antibodies on rat, marmoset, and human brain tissue. Concurrently, we develop protocols for improved tissue clearing and whole-mount imaging.